<p>HEK293T were cotransfected with CD14 and/or CD36 along with TLR2/1 (A) or TLR2/6 (B). The total amount of DNA in each transfection was kept constant by adding empty expression vector. The HEK293T cells were stimulated with rPCN (1.25 µg/mL), which was previously incubated with polymyxin to neutralize LPS. The positive controls were Pam3CysSK4 (P3C) for TLR2/1 and FSL-1 for TLR2/6. Medium was used as negative control for cell stimulation (white bars). Results are representative of three independent experiments. Statistical differences were assessed by comparing the response of cells lacking one of the co-receptors to the response of cells expressing both co-receptors, under similar stimuli. Values are the mean ± S.D. *** p<0.001.</p
