Differential gene expression analysis of retina RNA-Seq from wild type, pre-tumor, and retinal tumor tissues.

Abstract

<p>A, B Heat map representations of differentially regulated transcripts associated with tumor initiation and progression. (A) Differential gene expression of 62 Mutation-Driver genes. Tumor suppressors are in green, oncogenes are in red. (B) Differential gene expression of genes required for photoreceptor and neural function, neural progenitor proliferation, and injury induced regeneration. Scale represents log2 fold change in gene expression. (C) Comparison of absolute fold change in gene expression levels for <i>ajap1</i>, <i>ascl1a</i>, <i>atf3</i>, <i>bysl</i>, <i>hbegfa</i>, and <i>insm1a</i> measured by RNA-Seq (upper panel) and qRT-PCR (lower panel). <i>Tg(flk1:RFP)is18/+</i> Pretumor (red bars) and <i>Tg(flk1:RFP)is18/+</i> Tumor (blue bars).</p