New Tyrosinase
Inhibitors from Paecilomyces gunnii
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Abstract
Through
screening 50 strains of entomopathogenic fungi and rescreening of
7 strains of Paecilomyces gunnii, a
methanol extract of liquid-cultivated mycelia of P.
gunnii was found to have the strongest tyrosinase
inhibitory activity. Preparative high-speed counter-current chromatography
(HSCCC) guided by high-performance liquid chromatography (HPLC)–electrospray
ionization (ESI)–high-resolution mass spectrometry (HRMS) was
employed for the isolation and purification of the active components,
and three new compounds with half inhibition concentration (IC<sub>50</sub>) of 0.11, 0.17, and 0.14 mM against diphenolase were obtained
from the extract, respectively. Their chemical structures were identified
by HRMS, one- and two-dimensional nuclear magnetic resonance (2D NMR)
spectroscopy as paecilomycones A, B, and C. Structure and activity
studies showed that the tyrosinase inhibition activities are positively
related to the number of hydroxyl groups on the paecilomycones