Effective
and Selective Cell Retention and Recovery
from Whole Blood by Electroactive Thin Films
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Abstract
Hematogenous metastatic spread causes
most cancer patient deaths.
Because circulating tumor cells (CTCs) are highly relevant to early
metastatic spread, the capture or detection of these cells provides
a diagnostic tool for patients with metastatic conditions. Herein,
we demonstrate a programmable electroactive multilayered material
platform with a smart electrically induced “switch”
that captures CTCs from biological plasma with high efficiency and
releases the captured cells flexibly. The released cells are still
viable and proliferative, which facilitates the detection of trace
levels of CTCs by amplification. Furthermore, the inherent rough characteristics
of the nanoparticle-composed interface can promote capture efficiency
and cell purification by integration with a simple microfluidic device.
This elegant, inexpensive, and versatile platform for cell sorting
and enrichment makes subsequent molecular and cell biological analysis
achievable. The strategy has broad implications for favoring fundamental
cancer biology research, for the diagnosis and monitoring of cancer
individually, and for advanced intervention based on blood purification