Characterization
of Metabolite Profiles of Leaves
of Bilberry (Vaccinium myrtillus L.)
and Lingonberry (Vaccinium vitis-idaea L.)
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Abstract
Leaves
of bilberry (Vaccinium myrtillus L.)
and lingonberry (Vaccinium vitis-idaea L.) are potential raw materials for food and health care products.
Targeted (HPLC-DAD, HPLC-MS, and GC-FID) and nontargeted (<sup>1</sup>H NMR) approaches were applied to study the metabolomic profiles
of these leaves. Chlorogenic acid was the major phenolic compound
in bilberry leaves and arbutin in lingonberry leaves. Flavonol glycosides
were another major group of phenolics in bilberry [5–28 mg/g
DM (dry mass)] and lingonberry (15–20 mg/g DM) leaves. Contents
of fatty acids were analyzed using GC-FID. The changes in the metabolomics
profile during the season were apparent in bilberry but not lingonberry
leaves. Negative correlation was found between the contents of lipids
and phenolics. The consistency between the key results obtained by
targeted and nontargeted analyses suggests nontargeted metabolomic
analysis is an efficient tool for fast screening of various leaf materials
