The effects of cAMP signaling modulators on gem counts in SMA fibroblasts.

Abstract

<p>GM03813 SMA fibroblasts were treated with differing doses of dbcAMP, epinephrine, forskolin, salbutamol or rolipram for 5 days (n = 3/dose/drug). The concentrations for each dose of each drug (in µM) are shown below the appropriate bars of each graph. The vehicle for dbcAMP is ddH₂O (black bars) while DMSO (grey) serves as vehicle for the remaining compounds. The number of SMN-positive nuclear gems was counted in 100 randomly selected nuclei. Gem count analysis was also completed in GM03814 carrier fibroblasts so as to compare the gem data in treated SMA fibroblasts to those observed in healthy cells. The gem count analysis was expressed as (<b>A</b>) the number of gems per 100 nuclei, (<b>B</b>) the proportion of cells containing gems and (<b>C</b>) the proportion of cells containing multiple gems. The asterisk (*) denotes a statistically significant (p≤0.05) difference between drug-treated cells and vehicle (either ddH₂O for dbcAMP or DMSO for epinephrine, forskolin, salbutamol or rolipram)-treated cells.</p