<p>In the Blitz biosensor analysis, the signal corresponds to the mass collected on the biosensor surface. Here we used Streptavidin-coated sensor surfaces that were loaded with biotinylated antibody (10 µg/ml for 120 seconds) followed by apoD (2 µg/ml for 300 seconds) or buffer-control (300 seconds). The surfaces were subsequently loaded with the LDL, HDL2 or HDL3 lipoprotein particles (100 µg/ml for 300 seconds). Between loading reagents, the sensors were rinsed for 30 seconds. The buffer used for all steps and dilutions was PBS, azide, 0.1% BSA. <b>A</b>) The green, black, grey and red lines represent experiments preloaded with anti-apoD D544. The blue line indicates the control preloaded with isotype control antibody 7B6 (anti-IFNγ). The green and blue lines indicate that ApoD was preloaded, while the black, grey, and red lines indicate the buffer controls. The panel shows the final step in which the lipoprotein particles bind to the antibody loaded with or without apoD. The green, black and blue lines show binding of LDL, the grey line shows binding of HDL3 and the red line shows binding of HDL2. <b>B</b>) The binding step of the biotinylated antibody was omitted. Green, black, red and grey lines indicate experiments that used D544-biotin, and the blue line indicates that E981-biotin (anti-apoE) was used. Subsequent reactions show the addition of HDL2 in red, HDL3 in grey, apoD in green and blue, or buffer in black to the preloaded antibodies. In the last step, LDL was loaded in all the conditions.</p
