<div><p>Transgenic <i>Catharanthus roseus</i> plants (transgenic Dhawal [DT] and transgenic Nirmal [NT]) obtained from the <i>Agrobacterium tumefaciens</i> and <i>Agrobacterium</i><i>rhizognenes</i>-mediated transformations, respectively, have been maintained <i>in vitro</i> for 5 years. Plants were studied at regular intervals for various parameters such as plant height, leaf size, multiplication rate, alkaloid profile and presence of marker genes. DT plant gradually lost the <i>GUS</i> gene expression and it was not detected in the fifth year while NT plant demonstrated the presence of genes <i>rol</i><i>A</i>, <i>rol</i><i>B</i> and <i>rol</i><i>C</i> even in the fifth year, indicating the more stable nature of <i>Ri</i> transgene. Vindoline content in the DT was two times more than in non-transformed control plants. Alkaloid and tryptophan profiles were almost constant during the 5 years. The cluster analysis revealed that the DT plant is more close to the control Nirmal plant followed by NT plant.</p></div
