A Highly Tunable System for the Simultaneous Expression
of Multiple Enzymes in <i>Saccharomyces cerevisiae</i>
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Abstract
Control of the expression levels
of multiple enzymes in transgenic
yeasts is essential for the effective production of complex molecules
through fermentation. Here, we propose a tunable strategy for the
control of expression levels based on the design of terminator regions
and other gene-expression control elements in <i>Saccharomyces
cerevisiae</i>. Our genome-integrated system, which is capable
of producing high expression levels over a wide dynamic range, will
broadly enable metabolic engineering and synthetic biology. We demonstrated
that the activities of multiple cellulases and the production of ethanol
were doubled in a transgenic yeast constructed with our system compared
with those achieved with a standard expression system