Comprehensive Analysis of the Mouse Brain Proteome
Sampled in Mass Spectrometry Imaging
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Abstract
On-tissue enzymatic digestion is
performed in mass spectrometry
imaging (MSI) experiments to access larger proteins and to assign
protein identities. Most on-tissue digestion MSI studies have focused
on method development rather than identifying the molecular features
observed. Herein, we report a comprehensive study of the mouse brain
proteome sampled by MSI. Using complementary proteases, we were able
to identify 5337 peptides in the matrix-assisted laser desorption/ionization
(MALDI) matrix, corresponding to 1198 proteins. 630 of these peptides,
corresponding to 280 proteins, could be assigned to peaks in MSI data
sets. Gene ontology and pathway analyses revealed that many of the
proteins are involved in neurodegenerative disorders, such as Alzheimer’s,
Parkinson’s, and Huntington’s disease