Thermoresponsive Cationic Copolymer Brushes for Mesenchymal
Stem Cell Separation
- Publication date
- Publisher
Abstract
Thermoresponsive,
cationic, copolymer brushes poly(<i>N</i>-isopropylacrylamide(IPAAm)-<i>co</i>-<i>N</i>,<i>N</i>-dimethylaminopropylacrylamide-<i>co</i>-<i>N</i>-<i>tert</i>-butylacrylamide(tBAAm))
and poly(IPAAm-<i>co</i>-3-acrylamidopropyl trimethylammonium chloride-<i>co</i>-tBAAm) were prepared on glass substrates through surface-initiated
atom transfer radical polymerization. Prepared copolymer brushes were
investigated as thermally modulated cell separation materials. Densely
packed cationic copolymer brushes were formed on the glass substrates,
and the positive charge density was modulated by controlling the composition
of cationic moieties and species. During observation of cell adhesion
and detachment properties on copolymer brushes, human bone marrow
mesenchymal stem cells (hbmMSC) exhibited thermally modulated cell
adhesion and detachment, while other bone-marrow-derived cells did
not adhere. Using these properties, hbmMSC could be purified from
mixtures of human bone-marrow-derived cells simply by changing the
external temperature. Therefore, the prepared cationic copolymer brush
is useful for separation of hbmMSC