A. EMSA experiments with protein extracts from S2 cells and adult <i>D</i>. <i>melanogaster</i> flies exploring labeled fragments of the <i>D</i>. <i>melanogaster hsp70Aa</i> and <i>S</i>. <i>singularior hsp70S3</i> and <i>S4</i> genes. 1—Control (25°C), 2—heat shock (37°C), 3—heat shock + preimmune serum, 4—super shift with anti-HSF. The arrow indicates the position of the HSF-HSE complex. B. Recombinant <i>D</i>. <i>melanogaster</i> GAF protein effectively binds to the <i>D</i>. <i>melanogaster hsp70</i> and <i>S</i>. <i>singularior hsp70S3</i> promoters with the experimental insertion of three GAGA elements (lanes 1 and 4) but not with the wild-type <i>hsp70S3</i> and <i>hsp70S4</i> promoters (lanes 2 and 3). The arrow indicates the position of the GAF-GAGA complexes.

Abstract

<p>A. EMSA experiments with protein extracts from S2 cells and adult <i>D</i>. <i>melanogaster</i> flies exploring labeled fragments of the <i>D</i>. <i>melanogaster hsp70Aa</i> and <i>S</i>. <i>singularior hsp70S3</i> and <i>S4</i> genes. 1—Control (25°C), 2—heat shock (37°C), 3—heat shock + preimmune serum, 4—super shift with anti-HSF. The arrow indicates the position of the HSF-HSE complex. B. Recombinant <i>D</i>. <i>melanogaster</i> GAF protein effectively binds to the <i>D</i>. <i>melanogaster hsp70</i> and <i>S</i>. <i>singularior hsp70S3</i> promoters with the experimental insertion of three GAGA elements (lanes 1 and 4) but not with the wild-type <i>hsp70S3</i> and <i>hsp70S4</i> promoters (lanes 2 and 3). The arrow indicates the position of the GAF-GAGA complexes.</p