<p>Transcript expression studies were analyzed by <i>in situ</i> hybridization using a LNA (locked nucleic acid) probe. C57BL/6 mouse brains were sectioned and treated with the corresponding LNA probes. Signals were visualized by an avidin-biotin complex using DY-light 488 streptavidin <b>(A-F)</b> or DAB (3,3’-diaminobenzidin) staining <b>(G-M)</b>. III = third ventricle; OT = optical tract; DY-light 488 streptavidin labeled samples are shown with a 40-fold, DAB stained sections are depicted with a 20-fold magnification. Bar scale in (B) equals 100 μm, bar scale in (K) equals 200 μm. <b>(A-F)</b>: mTaar5 expression; <b>(A), (C)</b> and <b>(E)</b> represent negative controls using a scrambled LNA probe showing homogenous staining. <b>(B), (D)</b> and <b>(F)</b> show expression of mTaar5 in arcuate nucleus (ARC), in ventromedial hypothalamus (VMH) and amygdala, respectively. <b>(G-M)</b>: mTaar1 expression, the brain regions of interest are highlighted by circles. <b>(G)</b>, <b>(I)</b> and (<b>L)</b> represent negative controls using a scrambled LNA probe. <b>(K)</b> and <b>(M)</b> show expression of mTaar1 in mice brains in VMH and amygdala, respectively. <b>(H)</b> No expression could be detected in the ARC.</p
