Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information
