Diels–Alder Click-Based Hydrogels for Direct
Spatiotemporal Postpatterning via Photoclick Chemistry
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Abstract
Click chemistry not only has been
applied to the design of hydrogel
scaffolds for 3D cell culture, but also is an efficient way for hydrogel
postfunctionalization and spatiotemporal patterning. To the best of
our knowledge, only azide–alkyne cycloaddition (SPAAC) has
been exploited by combining photoinitiated thiol–ene click
reaction to realize the 3D patterning of hydrogels. In this work,
the cyclohexene derivative, which “clicked” by functional
groups between furyl and maleimide, were successfully functionalized
by thiol-modified molecules or peptides through thiol–ene click
reaction. It illustrates a hydrogel that formed via Diels–Alder
(DA) click chemistry between furyl-modified hyaluronic acid and bimaleimide
functional PEG molecule can be allowed for the directly photoactivated
thiol–ene chemistry for hydrogel spatiotemporal patterning.
Since the cyclohexene derivatives produced by DA reaction can be employed
in all subsequent 3D network patterning by using photoclick reactions,
it suggests a new way to design and postfunctionalize all of the DA
click-based hydrogels with specific regional bioactive cues