One-Photon and Two-Photon Sensing of Biothiols Using
a Bis-Pyrene-Cu(II) Ensemble
and Its Application To Image GSH in the Cells and Tissues
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Abstract
Glutathione
(GSH), cysteine (Cys), and homocysteine (Hcy) are three
major biothiols, which play key roles in various biological systems.
Accordingly, the development of imaging probes has been actively studied.
We report a new pyrene derivative <b>1</b>, which showed large
fluorescence quenching with Cu<sup>2+</sup> at pH 7.4. The ensemble <b>1</b>-Cu<sup>2+</sup> was applied to detect biothiols. Among the
various amino acids, GSH, Cys, and Hcy induced distinct turn-on fluorescence
changes. The <b>1</b>-Cu<sup>2+</sup> ensemble was further applied
for GSH detection in living cells