Characteristics of the microsatellite loci, including the type of fluorescent dye (Dye) and the mixing ratio of fluorescent and non-fluorescent forward primers (Fl: Non-fl), the observed number of alleles (<i>N</i><sub>A</sub>), observed (<i>H</i><sub>O</sub>) and expected (<i>H</i><sub>E</sub>) heterozygosity, probability of exclusion when one parent is known (<i>P</i><sub>EX</sub>), and estimated null allele frequency by locus for the 60 individual <i>Castanea crenata</i> trees at the Field Research Center, Tohoku University, Miyagi, Japan.
<p>These parameters were calculated using CERVUS 3.0.3 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120393#pone.0120393.ref054" target="_blank">54</a>].</p><p>*Overall combined exclusion probability</p><p>Characteristics of the microsatellite loci, including the type of fluorescent dye (Dye) and the mixing ratio of fluorescent and non-fluorescent forward primers (Fl: Non-fl), the observed number of alleles (<i>N</i><sub>A</sub>), observed (<i>H</i><sub>O</sub>) and expected (<i>H</i><sub>E</sub>) heterozygosity, probability of exclusion when one parent is known (<i>P</i><sub>EX</sub>), and estimated null allele frequency by locus for the 60 individual <i>Castanea crenata</i> trees at the Field Research Center, Tohoku University, Miyagi, Japan.</p
