<p>(A-C) When compared to <i>WT</i>, In situ hybridization and real-time quantitative PCR for <i>Wnt9b</i> demonstrates <i>Wnt9b</i> mRNA expression is significantly reduced (1.00 versus 0.29, p=0.008) in E14.5 <i>β-cat<sup>S-/-</sup></i> kidneys. (D-I) In situ hybridization and Real-time quantitative PCR for <i>Ret</i> and <i>Wnt11</i> demonstrated no differences in mRNA expression between <i>WT</i> and <i>β-cat<sup>S-/-</sup></i> at E14.5. (J-K) Histological analysis of <i>β-cat<sup>GOF-S</sup></i> mutant kidneys demonstrate a marked increase in condensing mesenchyme population when compared to <i>WT</i> at E14.5. (L-N) In situ hybridization and quantitative PCR demonstrate <i>Wnt9b</i> expression in <i>β-cat<sup>GOF-S</sup></i> kidneys was significantly increased (1.02 versus 3.102, p=0.021) as compared to <i>WT</i> at E14.5. (scale bar = 50 μm, rc-renal capsule, cm= condensing mesenchyme, ub = ureteric epithelium).</p
