<div><p>A new citromycetin analogue, ascomycotin A (<b>1</b>), together with eight known compounds, wortmannilactone E (<b>2</b>), orcinol (<b>3</b>), orsellinic acid (<b>4</b>), isosclerone (<b>5</b>), (3<i>R</i>,4<i>S</i>)-( − )-4-hydroxymellein (<b>6</b>), diorcinol (<b>7</b>), chaetocyclinone B (<b>8</b>) and 2,5-dimethoxy-3,6-di(<i>p</i>-methoxypheny1)-1,4-benzoquinone (<b>9</b>), was isolated from the fungal strain <i>Ascomycota</i> sp. Ind19F07, which was isolated from the deep sea sediment of the Indian Ocean. The structures of the compounds were established by spectroscopic data including 1D and 2D NMR and HR-ESI-MS. Compounds (<b>1</b>–<b>9</b>) were evaluated for antibacterial activity.</p></div