Expression of JHP0290 homologues in different <i>H</i>. <i>pylori</i> strains.

Abstract

<p>Whole cell lysate <b>(A)</b> and culture broth (Supernatant) from equal number of cells <b>(B)</b> of various <i>H</i>. <i>pylori</i> strains as indicated in figure legends were immunoblotted with anti-JHP0290 antibody. Blot shown is representative of results obtained in more than five independent experiments. The graph shows western blot band intensities quantified by the ImageJ software. <b>(C)</b> Sequence homology analysis of JHP0290 homologues in <i>H</i>. <i>pylori</i> strains J99 (JHP0290), 26695 (HP0305), P12 (HPP12_HP0304) and HPAG1 (HPAG1_HP0307) using Clustal Omega software (EMBL-EBI). N-terminal 1–17 amino acids were identified as signal peptide by the SignalP 4.1 software (ExpPASy Bioinformatics Resource Portal). Conserved Cysteine at position 162 is marked by an arrow.</p