<p>A. aegypti <i>(</i><b><i>A</i></b><i>and</i><b><i>C</i></b><i>) and</i> A. albopictus <i>(</i><b><i>B</i></b><i>and</i><b><i>D</i></b><i>) were</i> orally infected with blood meals (BM) containing 7.1 to 7.4 log<sub>10</sub> pfu/ml of recombinant D4 or D4-275-184 virus, respectively, and processed at 14 dpi. (<b>A</b> and <b>B</b>) Each point represents virus titer in individual mosquito (n = 18 for <i>A</i>. <i>aegypti</i> exposed to D4, and n = 24 mosquitoes for each other virus). Horizontal line represents an average titer for all mosquitoes. The dashed line indicates the limit of the assay detection [0.2 log<sub>10</sub> pfu/mosquito]. P-values comparing mean titers of D4 and miRNA targeted viruses were calculated using unpaired one-tailed Student's t-test and adjusted using Bonferroni correction method to account for multiple comparisons. (<b>C</b> and <b>D</b>) Percentage of mosquitoes that became infected (blue) or developed a disseminated infection (orange) with each virus. Differences in infection and dissemination frequencies were compared between D4 and miRNA targeted viruses using one-tailed Fisher’s exact test. P-values were adjusted using Bonferroni correction method to account for multiple comparisons.</p
