Fine structure of <i>Eleutheroschizon duboscqi</i> mature trophozoites.

Abstract

<p><b>A.</b> Mature trophozoite transforming into a gamont stage. TEM. <b>B.</b> Detailed view of the annular joint point and a well-developed fascicle of filaments. TEM. <b>C.</b> The view of mitochondria and a micropore (white circle) at the attachment site. The inset shows the micropore in detail. TEM. <b>D.</b> Higher magnification of the caudal region. TEM <b>E.</b> Two partially detached, mature trophozoites. SEM. <b>F.</b> The attachment site of a partially detached, mature trophozoite with well-developed fascicles and short filaments. SEM. <b>G.</b> Diagonal section of the apical part of a mature trophozoite. TEM. <b>H-I.</b> Craters left after detachment of mature trophozoites with well-developed attachment fascicles. Flat holes organised in one circle correspond to the developing lobes. SEM. <b>J.</b> A crater left after a trophozoite of more advanced stage as indicated by the presence of one circle of deep holes corresponding to well-developed lobes and one extra lobe starting the formation of a second circle. SEM. <i>a—</i>parasite amylopectin, <i>black arrow</i>—PS, <i>black arrowhead—</i>parasite plasma membrane, <i>black asterisk</i>—space between the parasite and PS, <i>black double/paired arrowheads</i>—parasite cytomembranes, <i>c</i>—parasite cytoplasm, <i>er</i>—parasite endoplasmic reticulum, <i>fa</i>—attachment fascicles, <i>fh—</i>holes in the host tissue left after the fascicles of the detached parasite, <i>fi—</i>short attachment filaments, <i>g</i>—glycocalyx, <i>h—</i>host cell, <i>l</i>—attachment lobe, <i>lh</i>—holes in the host tissue left after the lobes of the detached parasite, <i>m</i>—parasite mitochondria, <i>mv</i>—host microvilli, <i>n—</i>parasite nucleus, <i>p</i>—parasite, <i>sf—</i>parasite subpellicular filaments, <i>white arrow</i>—host cell plasma membrane, <i>white arrowhead</i>—dense band, <i>white asterisk</i>—empty attachment site, <i>white double arrowhead</i>—base of the PS.</p