<i>B</i>. <i>melitensis</i> oral infection results in an increase of CLN CD11b^high cells expressing either F4/80, Ly6c or both.

Abstract

<p>CLN from mice orally infected with 10⁹<i>B</i>. <i>melitensis</i> per mouse for 15 days were prepared for flow cytometry. Total CLN cell numbers were analyzed for the respective percentages <b>(A)</b> or absolute numbers <b>(B)</b> of dendritic cells (F4/80^-CD11c^highMHCII^int and F4/80^-CD11c^intMHCII^high), CD11b^high macrophages/monocytes (F4/80^-Ly6c⁺, F4/80⁺Ly6c⁺ and F4/80⁺Ly6c^-) and neutrophils (CD11b^highLy6G⁺). <b>(C)</b> shows a representative contour plot of respective populations from a mock-infected or <i>Brucella</i>-infected mouse on CD19^-Ly6G^-CD11b^high (F4/80 vs. Ly6c) or CD19^-CD11b^low/intF4/80^-NK1.1^- cells (CD11c vs. MHCII). Populations shown in (A) were analyzed for their median fluorescence of <b>(D)</b> CD11b, <b>(E)</b> CD11c and <b>(F)</b> MHCII. Data represent mean and SEM of pooled results from two independent experiments with a total of 8 (mock-infected) and 9 (infected) mice per group. * p ≤ 0.05 as compared to respective mock infected control.</p