<p>CLN from mice orally infected with 10<sup>9</sup><i>B</i>. <i>melitensis</i> per mouse for 15 days were prepared for flow cytometry. Total CLN cell numbers were analyzed for the respective percentages <b>(A)</b> or absolute numbers <b>(B)</b> of dendritic cells (F4/80<sup>-</sup>CD11c<sup>high</sup>MHCII<sup>int</sup> and F4/80<sup>-</sup>CD11c<sup>int</sup>MHCII<sup>high</sup>), CD11b<sup>high</sup> macrophages/monocytes (F4/80<sup>-</sup>Ly6c<sup>+</sup>, F4/80<sup>+</sup>Ly6c<sup>+</sup> and F4/80<sup>+</sup>Ly6c<sup>-</sup>) and neutrophils (CD11b<sup>high</sup>Ly6G<sup>+</sup>). <b>(C)</b> shows a representative contour plot of respective populations from a mock-infected or <i>Brucella</i>-infected mouse on CD19<sup>-</sup>Ly6G<sup>-</sup>CD11b<sup>high</sup> (F4/80 vs. Ly6c) or CD19<sup>-</sup>CD11b<sup>low/int</sup>F4/80<sup>-</sup>NK1.1<sup>-</sup> cells (CD11c vs. MHCII). Populations shown in (A) were analyzed for their median fluorescence of <b>(D)</b> CD11b, <b>(E)</b> CD11c and <b>(F)</b> MHCII. Data represent mean and SEM of pooled results from two independent experiments with a total of 8 (mock-infected) and 9 (infected) mice per group. * p ≤ 0.05 as compared to respective mock infected control.</p