<p>A comparison of images after stepwise injections of a SERS nanoparticle cocktail. Using an endoscope, the injection was made into the esophageal mucosal layer of a fresh intact postmortem pig. The esophagus was then scanned using our device. (a-c) Cylindrical three-dimensional reconstruction of ratiometric signal intensities of S493, S440, and S420 from the stepwise-mixture injection site. A total of 8,000 pixels (80 rev x 100 pix/rev) were acquired in a period 80 seconds (1 rev/s). (a) Ratiometric signal intensity of S493. (b) Ratiometric signal intensity of S440. (c) Ratiometric signal intensity of S420. (d) The center values of each bar in the bar graph are the average of ratiometric values for each of the respective SERS flavors where the value is non-zero (<i>n</i> = 123 for S440 equimolar and S420 equimolar, and <i>n</i> = 128 for S440 step-wise and S420 step-wise). The error bars are standard errors of the mean. (e) Cylindrical three-dimensional ratiometric image of S420 (from (c)) superimposed onto the white-light endoscope image of the esophagus. The device can be seen in the upper left corner with the scan mirror and window.</p
