3D imaging of DNA methylation in single interphase nuclei.

Abstract

<p>Immunofluorescence with a specific antibody against 5-methylcytosine was performed in rice root sections of ‘Pokkali’ (A-F) and ‘IR29’ (G-L) 14-days-old seedlings in control conditions or after 24h of exposure to salinity stress (200 mM NaCl). Bar = 10 μM. (M) Rice root-tip section obtained with a vibratome, and stained with DAPI. (N) Fluorescence intensity was calculated using Image J. The values obtained are shown as arbitrary units (AU).</p

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