Knockdown of LDHB induces the mitochondrion-dependent apoptosis pathway.

Abstract

<p>KB cells were transfected with scrambled or LDHB siRNA and then treated with DMSO or 10 nM taxol for 24 h. (<b>A</b>) The mitochondria and the cytoplasm were separated and measured for cytochrome C by western blotting. COX IV was used as a marker for mitochondria. (<b>B</b>) Mitochondrial cytochrome C was measured by FACS analysis. (<b>C</b>) Cell lysates were prepared for western blotting. GAPDH was used as a loading control.</p

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