<p>(<b>A</b>) Human phosphokinase array. Neutrophils were stimulated with CoMCont or CoMTB. Representative blot from <i>n</i> = 4 healthy donors. Red circle highlights increased AMPKα2 phosphorylation in CoMTB-stimulated cells, with densitometric analysis of components of AMPK pathway below. (<b>B</b>) CoMTB stimulation phosphorylates AMPKα1/2 analyzed by western blotting and gel densitometry. Neutrophils were stimulated for 30 minutes. Bars represent mean ± s.e.m from <i>n</i> = 3 donors. (<b>C</b>) Neutrophils were infected with <i>M</i>.<i>tb</i> MOI of 10 and cell lysates immunoblotted for phospho-AMPKα (T172) at defined time points. <i>M</i>.<i>tb</i> caused maximal phosphorylation at 120 mins. (<b>D</b>) Compound C (Comp C) pre-incubation for 30 minutes before CoMTB stimulation suppresses neutrophil MMP-8 secretion at 4 hours. (<b>E</b>) Compound C (Comp C) was pre-incubated for 30 minutes before stimulation with CoMCont or CoMTB for 24 hours with MMP-8 gene expression analyzed by real-time PCR normalized to GAPDH. Bars represent mean ± s.d. of an experiment performed in biological triplicates on at least 2 occasions. *<i>P</i><0.05, ***<i>P</i><0.001. Analysis was performed using one-way ANOVA with Tukey’s post-test.</p
