<p><b>[A]</b> Cell culture was seeded at concentration of 25,000–33,000 cells/cm<sup>2</sup> at day 0 (1.7 x 10^6 cells per 10cm dish). On days 1, 2 and 4 the inhibitors PD0325901 and SB431542 were applied at concentrations 0.5 μM and 2 μM. Cultures were three times replated (1:3 ratio) as indicated by a triple arrow. At day 25, the first colonies positive for alkaline phosphatase were detected (brown bar) and MEF culture medium was replaced by LIF–containing ESM medium (blue bar). In parallel experiments, fibroblast cultures were treated with mir294 mimics (days 1, 3 and 7) and p53 inhibitor (days 4 and 6). <b>[B]</b> Combinations of inhibitors for chiPSc reprogramming as applied on individual plates (experimental setup 1–9) with the number of resulting morphologically distinguishable and phosphatase positive iPSc-like colonies from four independent experiments in each setup.</p
