Calcein-acetoxymethyl ester (CAM), which exclusively stains live cells, was used to analyze cell survival.

Abstract

<p>(A) Control cells were CAM positive (green). Original magnification: 200x. (B) HOK were seeded in multidishes at different concentrations and incubated for two hours to ensure attachment. The CAM reagent was added to the cells for one hour, and CAM fluorescence was measured with a microplate fluorometer (excitation/emission filter pair 485/538nm).</p