Immunological detection of co-localization of RIC8 with Gα<sub>i1/2</sub>, LGN and NuMA proteins during oocyte development.

Abstract

<p>(<b>A-C</b>; <b>G-L</b>) Mouse oocytes at GV or (<b>D-F</b>) GVBD stages were double-labeled with RIC8 antibody (green) and NuMA, LGN or Gα<sub>i1/2</sub> antibodies respectively (red). DNA was stained with DAPI (blue). (<b>a-l</b>) Higher magnification of the region of chromatin. Dotted white line indicates the borders of oocyte. White arrowheads point RIC8 foci at nucleolus and on chromatin. Abbreviations: GV, germinal vesicle; GVBD, germinal vesicle breakdown. Scale bar: 10 μm.</p

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