<p>(<b>A, B</b>) RIC8 protein (green) expression in mouse oocytes was downregulated by microinjection of <i>Ric8</i> siRNA. DNA was stained with DAPI (blue). (<b>C</b>) Downregulation of <i>Ric8</i> mRNA expression with siRNA in microinjected oocytes quantified by qRT-PCR. (<b>D</b>) The relative intensity of RIC8 immunofluorescence signal measured by AutoQuant X3. (<b>E, F</b>) Localization of Gα<sub>i1/2</sub> (red) in mouse oocytes treated with <i>Ric8</i> and control siRNA at meiosis I. DNA was stained with DAPI (blue). (<b>G</b>) The relative intensity of Gα<sub>i1/2</sub> immunofluorescence signal measured by AutoQuant X3. Abbreviations: Cx, cortex. *<i>P</i> ˂ 0.05, **<i>P</i> ˂ 0.01; Students <i>t</i> test. Error Bars represent ±SEM scores. Scale bar: 10 μm.</p
