Combination therapy with InsB<sub>9–23</sub>, IGRP<sub>206–214</sub> and GAD<sub>524–543</sub> is less efficacious in promoting syngeneic islet transplant tolerance in NOD mice.
<p>A, diabetic NOD mice were transplanted with syngeneic islets. Recipients were treated with a mix of 3 islet peptides, insB<sub>9–23</sub>, IGRP<sub>206–214</sub> and GAD<sub>524–543</sub>/IFA and monitored for graft engraftment. Graph shows the percentage of islet graft survival after transplantation. Control, PBS/IFA-treated, mice were pooled from different experiments and used as reference in all experiments (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127631#sec002" target="_blank">Materials and Methods</a>). B, correlation between blood glucose levels at the time of transplant and the number of days of syngeneic islet engraftment for all mice. Survival curves were compared with the log-rank test. Correlation was done with Pearson coefficient. <i>P</i> and R<sup>2</sup> values are indicated in the graphs.</p
