Lamin A/C mislocation in mutant-transfected HEK 293 cells.

Abstract

<p>Transfection was performed using (A) GFP, (B) pEGFP-N1-LMNA, (C) pEGFP-N1-LMNA-R48P, (D) pEGFP-N1-LMNA-R249W, (E) pEGFP-N1-LMNA-I373V, or (F) pEGFP-N1-LMNA-I497_E536del. Lamin A/C of the mutants (C–F) is distributed in clusters and is mislocated compared with that of the wild type (B).</p