<p>The chemical composition of the essential oil obtained from aerial parts of <i>Nepeta graciliflora</i> was analysed, for the first time, by GC–FID and GC–MS. A total of 27 compounds were identified, constituting over 91.44% of oil composition. The oil was strongly characterised by sesquiterpenes (86.72%), with <i>β</i>-sesquiphellandrene (28.75%), caryophyllene oxide (12.15%), <i>α</i>-bisabolol (8.97%), <i>α</i>-bergamotene (8.51%), <i>β</i>-bisabolene (6.33%) and <i>β</i>-Caryophyllene (5.34%) as the main constituents. The <i>in vitro</i> activity of the essential oil was determined against four micro-organisms in comparison with chloramphenicol by the agar well diffusion and broth dilution method. The oil exhibited good activity against all tested organisms.</p
