Determination of oligomeric status of HpDprA and its domains.

Abstract

<p>Elution chromatograms of purified HpDprA at 2 mg/ml and 1 mg/ml <b>(A)</b> concentrations are depicted. Similarly, the size exclusion profiles of HpRF at 2 mg/ml at 1 mg/ml <b>(B)</b> and HpDML1 at 8 mg/ml at 2 mg/ml <b>(C)</b> are depicted. <b>(D)</b> Table shows elution volume (V<sub>e</sub>) and corresponding molecular weight of the elution peaks shown in (<b>A, B</b> and <b>C</b>). Elution was monitored using UV absorbance at 230 nm wavelength. The column was equilibrated in the same buffer as the protein samples (1X PBS). Total 500 µl sample was injected for each chromatophic experiment. The apparent molecular mass was calculated from elution volume using molecular weight standards as described in materials and methods.</p

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