Dual myeloid enhancer and T-cell repressor activity of element -25.

Abstract

<p>A) Diagram of element -25 and deletion constructs. 5’ T-cell repressor and 3’ myeloid enhancer modules of element -25 (-25 el.) are coloured in orange and red, respectively. The presence of the conserved 5’ RUNT (5’ R), 3’ RUNT and GATA (3’ R/G) motifs is indicated. Three deletion constructs (del -25 el., 5’del -25 el. and 3’del -25 el.) were produced by restriction enzyme digestion using <i>BglII</i> (position 445) and <i>NheI</i> (positions 878 and 1388). B, C) Independent myeloid enhancer and T-cell repressor activities of 2 modules within element -25. Deletion constructs were stably transfected together with either <i>LMO2</i> proximal (pPex) or intermediate (mdp) promoter elements in myeloid progenitors 416B (B) and T-cells Molt4 (C), respectively. In all cases, mean and standard error of the means (SEM) for at least two independent stable transfections (each one performed at least in triplicate) are shown. Values are expressed relative to empty vector, pGL2 basic. **P < 0.01; ***P < 0.001; ns, not significant.</p