Light-Activated Proteolysis for the Spatiotemporal
Control of Proteins
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Abstract
The regulation of proteolysis is
an efficient way to control protein
function in cells. Here, we present a general strategy enabling to
increase the spatiotemporal resolution of conditional proteolysis
by using light activation as trigger. Our approach relies on the auxin-inducible
degradation system obtained by transposing components of the plant
auxin-dependent degradation pathway in mammalian cells. We developed
a photoactivatable auxin that acts as a photoactivatable inducer of
degradation. Upon local and short light illumination, auxin is released
in cells and triggers the degradation of a protein of interest with
spatiotemporal control