Splenocyte proliferation assay for the bioactivity of membrane-bound IL-18 and/or GM-CSF.

Abstract

<p>Splenocytes were harvested from mock-transduced CT26 immunized Balb/C mice, and were cultured with CT26/IL-18, CT26/GM-CSF, and CT26/GM-CSF/IL-18 cells for the indicated times. ATPlite luminescence assay was performed to measure the splenocytes proliferation.</p