<p>HPDE or Colo357 cells incubated with Nrf2 or control siRNA for 48h were further treated for 24h with tBHQ or TGF-β1, either alone or in combination, or were left untreated. Then, either nuclear extracts (n.e.) or total cell lysates (t.c.l.) were analysed by westernblot (<b>A,C</b>), or RNA samples were analysed by real-time PCR (<b>B,D</b>) for the expression of Slug, L1CAM, E-cadherin or vimentin. Lamin-A/C and Hsp90 were used as loading controls for the westernblots of nuclear extracts and total cell lysates, respectively (<b>A,C</b>), and for normalization of Slug, L1CAM, E-cadherin and vimentin mRNA level TBP was analysed in parallel (<b>B,D</b>). Either a representative result from three independent experiments (<b>A,C</b>) or the mean ± SD from six independent experiments (<b>B,D</b>) are shown, *p<0.05 (+tBHQ versus–tBHQ). (<b>A,C</b>) a densitometric band intensity evaluation is provided in Figs. A and B in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132978#pone.0132978.s004" target="_blank">S4 File</a>.</p
