<p>(a) Fungal colony-forming units assessed using the culture plating method. All values are the means ± SD (n = 3). *<i>P</i> < 0.05 vs 0% group. (b) Similarity dendrogram of the fungal banding patterns of the soil samples. The scale for 0.49–1.00 was the similarity coefficient among the samples. DGGE fingerprints of the 18S rDNA sequences amplified using the primer pair Fung-GC/NS1 from soil-extracted community DNA. Lanes 1, 2 and 3 show the results of soil samples treated without salecan (C); lanes 4, 5 and 6 show the results of 0.02% salecan-treated samples; and lanes 7, 8 and 9 show the results of 0.2% salecan-treated samples. Five changed DGGE bands (F1, F2, F3, F4, F5) are marked by vertical black lines. (c) The phylogenetic relationship between the 18S rRNA gene sequences from the soil samples and related organisms from the GenBank database. The dendrogram was generated using the neighbor-joining method with 1000 bootstrap resamplings. The scale bar represented 0.5, estimated nucleotide changes per sequence position.</p
