Knockdown of TLR4 in A549 cells suppresses heme-induced intracellular ROS generation.

Abstract

<p>Following 24 hour transfection with both HO-1 and TLR4 siRNAs the cells were challenged with 100 μM heme for a further 24 hours. The cells were then detached with 0.05% trypsin and stained with 10 μM DCFDA at 37°C for 30 minutes. ROS was measured using flow cytometry. Data are means ± SEM of 3 independent experiments. *p≤0.05.</p