Figure S1. Male and female zebrafish were exposed separately to 25 nM E2 for 24 h and the brain tissue was isolated and dissected into three regions. RNA was isolated from individual samples followed by cDNA synthesis and qRT-PCR analysis. Statistical significance was determined as outlined in the materials and methods section. Statistically significant differences were determined using Student’s t test (*p < 0.05; **p < 0.01; *** p < 0.001). n = 4. Figure S2. Male and female zebrafish were exposed separately to 25 nM E2 for 24 h and the brain tissue was isolated followed by cDNA synthesis and qRT-PCR analysis. Statistical significance was determined as outlined in the materials and methods section. Statistically significant differences were determined using Student’s t test (*p < 0.05; *** p < 0.001). n = 4. Figure S3. Male and female zebrafish were exposed separately to 25 nM E2 for 24 h and the brain tissue was isolated followed by cDNA synthesis and qRT-PCR analysis. Statistical significance was determined as outlined in the materials and methods section. Statistically significant differences were determined using Student’s t test (*p < 0.05; **p < 0.01; *** p < 0.001). n = 4
