Comparative Biochemical and Proteomic Analyses of
Soybean Seed Cultivars Differing in Protein and Oil Content
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Abstract
This study develops differential
protein profiles of soybean (Glycine max) seeds (cv. Saedanbaek and Daewon) varying
in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) content using
protamine sulfate (PS) precipitation method coupled with a 2D gel
electrophoresis (2DGE) approach. Of 71 detected differential spots
between Daewon and Saedanbaek, 48 were successfully identified by
MALDI-TOF/TOF. Gene ontology analysis revealed that up-regulated proteins
in Saedanbaek were largely associated with nutrient reservoir activity
(42.6%), which included mainly seed-storage proteins (SSPs; subunits
of glycinin and β-conglycinin). Similar results were also obtained
in two cultivars of wild soybean (G. soja cv. WS22 and WS15) differing in protein content. Western blots confirmed
higher accumulation of SSPs in protein-rich Saedanbaek. Findings presented
and discussed in this study highlight a possible involvement of the
urea cycle for increased accumulation of SSPs and hence the higher
protein content in soybean seeds