LASIC: Light Activated Site-Specific Conjugation of
Native IgGs
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Abstract
Numerous biological applications,
from diagnostic assays to immunotherapies,
rely on the use of antibody-conjugates. The efficacy of these conjugates
can be significantly influenced by the site at which Immunoglobulin
G (IgG) is modified. Current methods that provide control over the
conjugation site, however, suffer from a number of shortfalls and
often require large investments of time and cost. We have developed
a novel adapter protein that, when activated by long wavelength UV
light, can covalently and site-specifically label the Fc region of
nearly any native, full-length IgG, including all human IgG subclasses.
Labeling occurs with unprecedented efficiency and speed (>90% after
30 min), with no effect on IgG affinity. The adapter domain can be
bacterially expressed and customized to contain a variety of moieties
(e.g., biotin, azide, fluorophores), making reliable and efficient
conjugation of antibodies widely accessible to researchers at large