NSE/APPsw transgenic mouse-derived brain slices treated with BDNF-depleted CM. (A) Western blot showed that hNSCs secreted high level of BDNF into the cultured medium (lane 1; CM), and anti-BDNF antibody-mediated immunoprecipitation effectively removed BDNF in CM (lane 2; BDNF-depleted CM). Another SDS-PAGE gel was in parallel performed with silver staining to verify even loading. (B) Brain slices treated with DMEM (Ctr), CM, BDNF-depleted CM. Western blot analysis of phosphorylated tau (AT180) and BACE1 (n = 3 per group, where n is the number of experiments). All data represent mean ± SEM. Error bars indicate ± SEM. Mann–Whitney U-test, *p < 0.05. (TIFF 2110 kb
