<p><b>(A)</b> Expanded trophoblast cells in <i>Rac</i>1<sup><i>d/d</i></sup> uteri on day 8 of gestation. <b>a:</b> Hematoxylin and Eosin (H & E) staining of uterine sections from <i>Rac1</i><sup><i>f/f</i></sup> and <i>Rac1</i><sup><i>d/d</i></sup> mice on day 8 of pregnancy. Three representative images of <i>Rac</i>1<sup><i>d/d</i></sup> uterine sections are shown. <b>b:</b> Quantitation of embryonic areas in H & E stained uterine sections of <i>Rac1</i><sup><i>f/f</i></sup> and <i>Rac1</i><sup><i>d/d</i></sup> mice on day 8 of pregnancy. Data represent mean ± SEM from six separate samples and were analyzed by non-parametric <i>t</i>-test. Asterisks indicate statistically significant differences (*<i>P < 0</i>.05). <b>(B)</b> Increased proliferation of trophoblast cells in the ectoplacental cone (EPC) of <i>Rac</i>1<sup><i>d/d</i></sup> uteri. Uterine sections from <i>Rac1</i><sup><i>f/f</i></sup> and <i>Rac1</i><sup><i>d/d</i></sup> mice on days 7 (panels a and b) and 8 of pregnancy (panels c and d) were subjected to IF using PCNA and cytokeratin 8 (KRT8) antibodies. AMD, MD, and E denote antimesometrial decidua, mesometrial decidua, and embryo respectively.</p
