In-Situ Grafting MPEG on the Surface of Cell-Loaded Microcapsules for Protein Repellency

Abstract

<div><p>The protein repelled alginate-graft-BAT/chitosan/MPEG-norbornene (A<sub>B</sub>CP<sub>N</sub>) hydrogel microcapsules were achieved by copper-free ‘click’ reaction between azides from BAT and alkylenes from norbornene. The MPEG modified polyelectrolyte microcapsules showed significant resistance to immune protein adsorption and good biocompatibility in vivo. Moreover, the mild reaction condition made it feasible that the microcapsules could be formed and modified <i>in situ</i> even when live cells were encapsulated, and precluded the damage cause by other voilent modifications methods to transplanted cells or tissues.</p></div

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