Sensitive
Detection of ssDNA Using an LRET-Based Upconverting
Nanohybrid Material
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Abstract
Water-dispersible,
optical hybrid nanoparticles are preferred materials
for DNA biosensing due to their biocompatibility. Upconverting nanoparticles
are highly desirable optical probes in sensors and bioimaging owing
to their sharp emission intensity in the visible region. We herein
report a highly sensitive ss-DNA detection based on an energy transfer
system that uses a nanohybrid material synthesized by doping NaYF<sub>4</sub>:Tm<sup>3+</sup>/Yb<sup>3+</sup> upconverting nanoparticles
(UCNPs) on silica coated polystyrene-<i>co</i>-acrylic acid
(PSA) nanoparticles (PSA/SiO<sub>2</sub>) as the donor, and gold nanoparticles
(AuNPs) decorated with Ir(III) complex as the acceptor. UCNPs tagged
on PSA/SiO<sub>2</sub> and the cyclometalated Ir(III)/AuNP conjugates
were then linked through the ss-DNA sequence. Sequential addition
of the target DNA to the probe molecular beacon complex resulted in
the separation of the optical nanohybrid material and the quencher,
leading to a measurable increase in the blue fluorescence emission
intensity. Our results have shown a linear relationship between the
fluorescence intensity and target DNA concentration down to the picomolar