Additional file 6: Figure S4. of Morphological change of CD4+ T cell during contact with DC modulates T-cell activation by accumulation of F-actin in the immunology synapse
Ca2+ responses in CD4+ T cells were measured and presented by △F/F. (A) The shape index change and Ca2+ signal in a CD4+ T cell whose morphology changed to elongated-flattened (top panel). (B) The shape index change and Ca2+ signal of a CD4+ T cell whose morphology changed to flattened (top panel). (C) The shape index change and Ca2+ signal in a resting T cell. (A-C) Ca2+ signalling was obtained every 10-s or 40-s. (D) Average Ca2+ responses of CD4+ T cells whose morphology changed to round-flattened or to elongated-flattened. (Data are shown as mean ± s.e.m., two-tailed Student’s t-test, ***p < 0.001). (E) Ca2+ response of a CD4+ T cell after it contacted DC pulsed OVA(323–339) in the presence of the cytochalasin D or not. (F) Average Ca2+ responses were measured in CD4+ T cells during the IS formation in the presence of cytochalasin D, nocodazole, TG or cytochalasin D and TG. (mean ± s.e.m, n = 25, three independent experiments), ***P < 0.001 (two-tailed Student’s t-test). (G) Average Ca2+ responses were measured in elongated and/ or CD4+ T cells during contact with DC in the presence of the cytochalasin D or TG treatment. (mean ± s.e.m, n = 25, three independent experiments), ** P < 0.01, ***P < 0.001 (two-tailed Student’s t-test). (H) Ca2+ response in a CD4+ T cell with TG stimulation and in a CD4+ T cell forming IS with TG pretreatment. (I) Ca2+ response of a CD4+ T cell with the cytochalasin D and TG treatment and a CD4+ T cells that formed IS with the cytochalasin D and TG pretreatment
