<p>Photoevoked excitatory post-synaptic currents were measured in patch-clamped muscle, during repeated photostimulation of cholinergic neurons expressing ChR2, at 0.5 Hz. <b>A)</b> Representative original current traces of wild type, compared to mutants affecting SV loading (<i>unc-17(e113)</i> vAChT), SV recycling (<i>unc-26(s1710)</i> synaptojanin, or Ca<sup>2+</sup> influx (<i>unc-2(ra612)</i> VGCC). <b>B)</b> Group data and statistical analysis, corresponding to the experiments in A. Wild type data is shown in black. Left panel: Mean inward currents (pA) ± SEM, in response to repeated stimulation with 10 ms blue light at 0.5 Hz. Right panel: Data was normalized to the first peak inward current [%]. Statistically significant differences were assessed by one-way ANOVA compared to wild type (* P < 0.05, ** P < 0.01, *** P < 0.001). For analysis of miniature PSC currents and frequencies of these strains, see <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135584#pone.0135584.s005" target="_blank">S5 Fig</a>.</b></p
